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Thermal denaturation of fresh frozen tissue enhances mass spectrometry detection of peptides

By Angela R.s. Kruse and others at
LogoVanderbilt University
Thermal denaturation (TD), known as antigen retrieval, heats tissue samples in a buffered solution to expose protein epitopes. Thermal denaturation of formalin-fixed paraffin-embedded samples enhances on-tissue tryptic digestion, increasing peptide detection using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS). We investigated the tissue-dependent effects of TD on peptide... Show more
July 9, 2024
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Thermal denaturation of fresh frozen tissue enhances mass spectrometry detection of peptides
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