Reengineering the Specificity of the Highly Selective Clostridium botulinum Protease via Directed Evolution

The botulinum neurotoxin serotype A (BoNT/A) cuts a single peptide bond in SNAP25, an activity used to treat a wide range of diseases. Reengineering the substrate specificity of BoNT/As protease domain (LC/A) could expand its therapeutic applications; however, LC/As extended substrate recognition ({approx}60 residues) challenges conventional approaches. We report a... Show more