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Optimization of a bacterial three-hybrid assay through in vivo titration of an RNA-DNA adapter-protein

By C. Wang and others at
LogoMount Holyoke College
Non-coding RNAs regulate gene expression in every domain of life. In bacteria, small RNAs (sRNAs) regulate gene expression in response to stress and are often assisted by RNA-chaperone proteins, such as Hfq. We have recently developed a bacterial three-hybrid (B3H) assay that detects the strong binding interactions of certain E.... Show more
January 26, 2021
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Optimization of a bacterial three-hybrid assay through in vivo titration of an RNA-DNA adapter-protein
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